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Application | Fiber Photometry

Full taper light collection

Lambda fibers are able to collect light all along their active area. Fluorescence is collected nearly homogeneously from large functional regions such as the cortex or the striatum in the mouse.
When combined with full taper light delivery, this property translates in a higher signal collected by a Lambda fiber with respect to a flat-cut fiber for similar illumination power density [1].

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 How to do 
 This mode of operation is straightforwardly obtained by replacing a standard flat fiber with a Lambda fiber* in a conventional fiber photometry experiment.
* based on the same or similar fiber type (i.e. core size and Numerical Aperture)

Application | Fiber Photometry

Site selective light collection

This mode of operation refers to the possibility to selectively record light from spatially-confined brain regions at arbitrary depth along the taper. Selectivity is obtained by controlling the position of the excitation, i.e. by site-selective light delivery, while the elicited fluorescence is collected by the whole taper. This is possible because fluorescence is generated only in the volume of tissue surrounding the taper sub-section selected for delivery of the excitation signal [1].

 How to do

 Site-selective fiber photometry with tapered fibers can be realized by complementing the ThetaStation with the Magic Box. See equipment   documentation for more information.

REFERENCES

 [1] F. Pisano, et al., “Depth-resolved fiber photometry with a single tapered optical fiber implant”, Nature Methods (2019)

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