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Application | Fiber Photometry

Whole-taper fiber photometry

Lambda fibers are able to collect light all along their active area. Fluorescence is collected nearly homogeneously from large functional regions such as the cortex or the striatum in the mouse.

When combined with whole-taper light delivery, this property translates in a higher signal collected from the Lambda fiber than from a flat fiber for similar illumination power density at the active optical surface [1].

HOW TO DO

This mode of operation is straightforwardly obtained by replacing a standard flat fiber with a Lambda fiber in a standard-configuration fiber photometry experiment.

tapered
Application | Optogenetics

Site-selective fiber photometry

This mode of operation refers to the possibility to selectively record from spatially-confined brain regions at arbitrary depth along the taper. Selectivity is obtained by controlling the position of the excitation, i.e. by site-selective light delivery, while the elicited fluorescence is collected by the whole taper. This is possible because fluorescence is generated only in the volume of tissue surrounding the taper sub-section selected for delivery of the excitation signal [1].

HOW TO DO

Site-selective fiber photometry with tapered fibers can be realized by complementing the ThetaStation (mettere link) with the Magic Box (mettere link). See equipment documentation for more information.

REFERENCES

 [1] F. Pisano, et al., “Depth-resolved fiber photometry with a single tapered optical fiber implant”, Nature Methods (2019)

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