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Application | Optogenetics

Whole-taper light delivery

This mode of operation is typically employed with Lambda fibers to target large volumes of tissue more homogeneously and efficiently than flat fibers [1]. Lambda fibers are typically inserted into the region to be controlled. Fortunately, the tapered profile with sub-micron tip diameter minimizes tissue displacement and insertion damage [1].

comparzione_fibra
How to do Whole-Taper light delivery

Whole-taper light emission can be achieved with any Laser or LED light source.
For correct operation, the Numerical Aperture (NA) of the light source should be at least equal to that of the tapered fiber (NA_source ≥ NA_fiber). Using a patch cable with the same NA of the tapered fiber is recommended.

Site-selective light delivery

Light emission can be restricted to sub-portions of the taper by the use of unconventional light delivery methods [1]. Emission profiles depend on the type of fiber as well as the actual light source employed.

NB: This feature can also be employed to correct for implantation errors or uncertainty. 

with Lambda fibers
How to do Site-Selective light delivery

This mode of operation is achieved by using OptogeniX dedicated equipment in combination with OptogeniX tapered fibers. 

See equipment documentation for more information.

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