Description
GalvoStation is a single channel opto-electronic laser device specifically designed to perform spatial selective Optogenetics and Fiber Photometry with OptogeniX Lambda Fibers.
GalvoStation allows to confine the light emitted by a Lambda Fiber at sub-sites of its active length. The active sub-site can be scanned arbitrarily along the fiber axis within the active length.
This feature can be exploted to illuminate different brain regions with depth resolution in order to i) record the photo-induced elctrophysiology signal at different depths with a multielectrode array [1], or ii) collect the fluorescence signal from different brain regions in a depth-resolved fiber photometry experiment [2].
Axial resolution of site selective light delivery/collection with Lambda fibers is represented by the sub-site length. To obtain higher repeatability among different fibers and to achieve a uniform emission length along the scanning range, the use of Lambda-plus Fibers is recommended (see details below).
GalvoStation can be equipped with one or two laser sources and optionally include an optical path for combined light delivery and collection with a single probe. Examples of options sets with related applications are listed in the following table.
| Laser wavelengths [nm] |
Collection band [nm] |
Application example |
| 405 + 473 | 500 – 550 | GCaMP based spatial selective Fiber Photometry with isosbestic control |
| 473 | N/A | ChR2 based spatial selective optogenetics |
| 405 + 561 | 600 – 650 | RCaMP based spatial selective Fiber Photometry with isosbestic control |
Lambda-plus Fibers
To obtain higher repeatability among different fibers and to achieve a uniform emission length along the scanning range, Lambda-plus Fibers are strongly recommended for use with the GalvoStation. Lambda-plus Fibers are Lambda Fibers selected with strict tolerance on both taper length and taper profile for a constant sub-site length among fibers of different batches. Visit the Lambda-plus Fibers shop page for an updated list of specs currently available and related axial resolution.
Customization
GalvoStation is designed with flexibility in mind: we welcome requests for customized configurations tailored to specific research needs beyond our standard options.
REFERENCES
[1] F. Pisanello, et al., “Multipoint-Emitting Optical Fibers for Spatially Addressable In Vivo Optogenetics“, Neuron (2014)
[2] F. Pisano, et al., “Depth-resolved fiber photometry with a single tapered optical fiber implant”, Nature Methods (2019)
